Floral classification of honey using liquid chromatography diode array detection China 2013

Floral classification of honey using liquid chromatography diode array detection China 2013

Floral classification of honey using liquid chromatography diode array detection China 2013

A high performance liquid chromatography–diode array detection–tandem mass spectrometry (HPLC–DAD–MS/MS) method for the floral origin traceability of chaste honey and rape honey samples was firstly presented in this study. Kaempferol, morin and ferulic acid were used as floral markers to distinguish chaste honey from rape honey. Chromatographic fingerprinting at 270 nm and 360 nm could be used to characterise chaste honey and rape honey according to the analytical profiles. Principal component analysis (PCA), partial least squares (PLS), partial least squares-discrimination analysis (PLS-DA) and soft independent modeling of class analogy (SIMCA) were applied to classify the honey samples according to their floral origins. The results showed that chaste honey and rape honey could be successfully classified by their floral sources with the analytical methods developed through this study and could be considered encouraging and promising for the honey traceability from unifloral or multifloral nectariferous sources.

Download Full Article